Summary of Position: Postdoc will engineer bile acid biosynthesis into a yeast strain. The UDCA biosynthetic pathway genes will be identified from various source organisms, synthesized and assembled into multi-gene constructs, and analyzed by LC-MS/MS of yeast fermentations. The successful candidate will be able to creatively improve on existing metabolic engineering methods while moving the project forward with regular progress. The postdoc will be expected to communicate their findings in an organized and profession manner through oral, poster, and written communications.
Essential and Other Functions: 40% Molecular Biology Building and testing multi-gene plasmids and integrating them to yeast; genome engineering of yeast; using state-of-the-art genome manipulation techniques
40% Analytical Chemistry Develop a robust and efficient pipeline for analyzing UDCA and intermediate pathway compounds via LC-MS/MS, and other analytical methods
20% Writing and presentation of data Formally communicate their data through written reports, slide presentations, and posters. The candidate is expected to communicate their productivity and research directions during weekly group meetings
Essential Qualifications PhD in Biological Engineering or equivalent field.
Preferred Qualifications: Experience with metabolic engineering in yeast.
Internal Number: 337051
About University of Minnesota, Twin Cities
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