The Sternberg Lab at Columbia University Irving Medical Center (www.sternberglab.org) is seeking a highly motivated Technician B to help manage daily operations in the laboratory, and to participate in ongoing research projects in collaboration with Dr. Sternberg and other members of the lab. The Sternberg Lab investigates the mechanisms of adaptive immunity in bacterial CRISPR-Cas systems using a combination of biochemical, structural, and biophysical approaches, and harnesses this information to advance novel tools with which to precisely engineer the genome. Founded in early 2018, the Lab presently comprises 4 PhD students and 3 undergraduates, and continues to grow.
The Technician will:
Be in charge of ordering and receiving
Be responsible for keeping lab inventories well-stocked and organized
Maintain the lab's buffer and media needs
Be responsible for glassware and plastic-ware washing
Help other lab members with molecular cloning
Help maintain the lab's plasmid DNA inventory and stocks
Requires a bachelor's degree in biochemistry, molecular biology, chemistry or related discipline and laboratory research experience
Applicant must have excellent organizational and communication skills, and should be comfortable reading primary research articles in the CRISPR biology/technology space.
A successful candidate will be able to complete tasks in a timely manner, with fine attention to detail, and be able to multitask effectively. Being interested to engage socially with the rest of the lab is a plus
The applicant must be a team player and enjoy/excel working collaboratively in groups.
Applicant should have strong knowledge of chemistry, biology, and microbiology, and should be proficient working with E. coli (solid and liquid media culturing, plasmid transformation, etc.).
Applicant must have experience with molecular cloning (e.g. PCR, agarose gel electrophoresis, restriction digestion/ligation and/or Gibson assembly) and must have excellent proficiency using micropipettes, running gels, setting up reactions using commercially available enzymes, and learning new techniques.
Equal Opportunity Employer / Disability / Veteran
Columbia University is committed to the hiring of qualified local residents.
Internal Number: 500403
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