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The Yadav lab is interested in understanding the neurobiology of developmental disorders such as autism and schizophrenia with a focus on dissecting the role of kinases in neuronal and synaptic development as well as understanding how mutations in these signaling molecules contribute to disease. Using cutting edge chemical-genetics, proteomics, imaging and induced pluripotent stem cell based approaches; the long-term goal of the lab is to dissect the signaling pathways that mediate synaptic and neuronal development in order to understand the molecular underpinnings of neurological disorders.
Learning and implementing molecular biology assays including DNA isolation, cloning, site directed mutagenesis and primer efficiency testing, analyzing the data and preparing reports
Perform biochemical assays such as western blots, protein purification and protein pull down assays under limited supervision
Duties will involve preparing and maintaining primary neuronal cultures
Management of cell lines and other common lab reagents, distribution to students and collaborators (including shipping logistics to outside institutions)
Implementing and continuously adapting wide range of standard operating procedures (SOPs) essential for lab functionality.
Contribute to preliminary results for grant writing and funding.
Assist in preparing, submitting and managing IACUC protocols, including amendments and well as renewals
Management duties include:
Implementing and enforcing various EH&S safety regulations for Laboratory Safety Plans, as well as IACUC regulation.
Training lab members in specific guidelines for lab duties and work (including use of EH&S restricted products and DEA drug compounds).
Being the lab Chemical Hygiene officer
Being the lab's representative for internal regulatory agencies such as IACUC, EH&S
Proficiency in complex ordering process
Directly assists PI with communications and compliance with external state and federal government agencies
Bachelor's Degree in biology, neuroscience, biochemistry, molecular biology or related fields and two years prior experience in molecular biology techniques such as cloning and DNA isolation required. Prior experience in cell culture required. Willingness to learn new techniques.
DESIRED: Prior experience in culturing and differentiation stem cells desirable. Experience in protein biochemistry is desirable.
Conditions of Employment: Appointment to this position is contingent upon obtaining satisfactory results from a criminal background check.
Application Process: The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Criminal Conviction History, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select
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